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Some herbs contained in the National Park include wood eye Flores (Albizia montana), needs (Homalanthus giganteus), tokotaka (Putranjiva roxburghii), Uwi RORA (Ardisia humilis), longgo steel (Drypetes subcubica), store Keo (Cyrtandra sp.) , Deo wood (Trema cannabina), and Kelo (Ficus villosa).

Flores National Park is the habitat of 19 endangered bird species including Flores pigeon (Treron florists), Wallacea owl (Otus silvicola), sweep the jungle-swinging (Rhinomyias oscillans), kancilan Flores (Pachycephala nudigula), junk dwarf (Pericrocotus lansbergei) , tesia Timor (Tesia everetti), opior crested (Lophozosterops dohertyi), opior half thick (Heleia crassirostris), chili gold (Dicaeum annae), kehicap Flores (sacerdotum Monarcha), bird honey Matari (Nectarinia solaris), and Flores eagle (Spizaetus florists).

Of the four species of mammals endemic to this park, which is frequently encountered are two mountain rat Rattus hainaldi.Di Bunomys naso and national parks can be found several animals such as the banteng (Bos javanicus javanicus), barking deer (Muntiacus muntjak nainggolani), mongoose (Pardofelis marmorata) , pangolin (Manis javanica), porcupine (Hystrix brachyura brachyura), and deer (Tragulus javanicus javanicus).
While the marine life around the island and the Cape of Darkness consists of 45 species of corals such as Halimeda macroloba, Chromis spp., Balistes spp., Zebrasoma spp., And Ypsiscarus ovifrons; 32 species of fish such as fish flag (Platax pinnate), fish conscious (Siganus lineatus), and barracuda (Sphyraena jello); 9 types of marine mollusk such as clams south (Tridacna derasa), triton trumpet (Charonia tritonis), and giant clams (Tridacna gigas).

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